Antimicrobial activity and biosynthesis of indole antibiotics produced by Xenorhabdus nematophilus

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چکیده

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Transformation of Xenorhabdus nematophilus.

The ability of Xenorhabdus nematophilus 19061/1 to be transformed by pHK17 plasmid DNA was studied and optimized. A number of factors, including culture conditions, stage of growth, transformation buffer pH, cation type and concentration required for the production of competency, washing, heat shock conditions, and cell-DNA ratio, were found to affect transformation significantly. On the basis ...

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BACKGROUND Xenocoumacin 1 (Xcn1) and Xenocoumacin 2 (Xcn2) are the main antimicrobial compounds produced by Xenorhabdus nematophila. Culture conditions, including pH, had remarkably distinct effects on the antimicrobial activity of X. nematophila. However, the regulatory mechanism of pH on the antimicrobial activity and antibiotic production of this bacterium is still lacking. RESULTS With th...

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ژورنال

عنوان ژورنال: Journal of General Microbiology

سال: 1993

ISSN: 0022-1287

DOI: 10.1099/00221287-139-12-3139